Biolegend, 682601, Purified anti-TOX Antibody, 25μg

TOX is a member of the high-mobility group (HMG) box superfamily of DNA-binding proteins and is originally identified as the thymic transcript expressed in CD4 + CD8 + double positive T-cells. TOX is also defined as part of a small subfamily of transcription factors consisting of TOX2, TOX3, and TOX4, which share conserved HMG-box sequences. TOX is highly upregulated in thymocytes undergoing positive selection and is required for the differentiation and development of various T-cell subsets, such as CD4 + T cells, regulatory T cells, and natural killer T cells (NKTs). TOX-deficient mice fail to develop lymph nodes and have significantly diminished numbers of CD4+ T-cells, NK cells, and ILC cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TOX recombinant protein (66-301 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The immunogen used to generate clone 6E6D03 is the N-terminus of Tox1, which is highly conserved among Tox family member proteins. Hence, this clone may recognize multiple Tox family members. Predicted MW for Tox family members: Tox 1 is 57.5 kD, Tox 2 is 49, 52, 53, 55 kD, Tox 3 is 63 kD and Tox 4 is 64, 66 kD.
Product Citations: Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID: AB_2566642 (BioLegend Cat. No. 682601) AB_2566643 (BioLegend Cat. No. 682602)
Structure: 562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.
Distribution: Nucleus.
Function: TOX is a transcription factor required for development of various T lymphocyte subsets.
Interaction: TOX interacts with ZDHHC17.
Biology Area: Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Spits H. 2015. Nat. Immunol. 16:594. 2. Seehus CR, et al. 2015. Nat. Immunol. 16:599. 3. Huang Y, et al. 2015. Blood 125:1435. 4. Aliahmad P, et al. 2012. Curr. Opin. Immunol. 24:173. 6. Di Santo JP. 2010. Nat. Immunol. 11:885. 7. Aliahmad P, et al. 2004. J. Exp. Med. 199:1089.
Gene ID: 9760
UniProt: View information about TOX on UniProt.org
Clone: 6E6D03
Regulatory Status: RUO
Other Names: Thymocyte Selection-Associated High Mobility Group Box, Thymus High Mobility Group Box Protein TOX, TOX1, KIAA0808
Isotype: Mouse IgG1, κ