Biolegend, 683502, Purified anti-HDAC7 Antibody, 100μg

Histone deacetylases (HDACs) regulate a variety of biological processes by deacetylating lysine residues in the N-terminal tails of core histones and non-histone proteins. HDAC7 is a class IIa histone deacetylase and its enzymatic activity is dependent on the interaction with HDAC3. HDAC7 is highly expressed in CD4 + CD8 + double-positive thymocytes and has been suggested to repress the expression of Nur77 through the transcription factor MEF2D. HDAC7 is mostly localized to the nucleus and can be sequestrated in the cytoplasm by interacting with 14-3-3 protein during T cell receptor activation. The nuclear export of HDAC7 leads to the expression of Nur77 and apoptosis. HDAC7 has been shown to regulate the maturation of osteoclast and osteoblast, as well as the development of muscle and blood vessel.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic HDAC7 peptide conjugated to KLH (1-17 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2572024 (BioLegend Cat. No. 683502)
Structure: 952 amino acids with a predicted molecular weight of 102.9 kD. Contains a histone deacetylase domain.
Distribution: Cytoplasm and the nucleus.
Function: HDAC7 mediates deacetylation of lysine residues in the N-terminal tails of core histones. It plays important roles in the regulation of T-cell differentiation and development of muscle and blood vessel.
Interaction: HDAC7 interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, FOXP3, KAT5, EDNRA, KDM5B, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Barneda-Zahonero B, et al. 2013. PLoS Genet. 9:e1003503. 2. Pham L, et al. 2011. J. Biol. Chem. 286:12056. 3. Li S, et al. 2011. Mol. Cancer 10:18. 4. Gao C, et al. 2010. Biochim. Biophys. Acta. 1803:1186. 5. Margariti A, et al. 2009. J. Cell. Sci. 122:460. 6. Parra M, et al. 2007. Genes Dev. 21:638.
Gene ID: 51564
UniProt: View information about HDAC7 on UniProt.org
Clone: 11C7C49
Regulatory Status: RUO
Other Names: Histone deacetylase 7, HD7, HDAC7a
Isotype: Mouse IgG1, κ