Biolegend, 684401, Ultra-LEAF™ Purified anti-human CD95 (FAS) Antibody, 100μg

FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells.  FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. The extracellular region of the FAS possesses three cystein-rich domains characteristic of the TNF superfamily, and the intracellular region includes a death domain (DD). The binding of FASL to FAS induces conformational changes in FAS, leading to recruitment of FADD and procaspase-8, and assembly of the death-inducing signalling complex (DISC). Subsequently, caspase 8 is released from DISC and induces activation of caspase 3 and 7, proteolysis of cellular components, and apoptosis. Biological effects not based on apoptotic cell death have also been described. The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC–lysosome-associated membrane protein (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. Human activated PBMC and tumor cell lines express full-length mRNA and several mRNA FAS variants derived by alternative splicing; deletion of an exon encoding the transmembrane domain results in a soluble FAS molecule that blocks apoptosis. Patients with systemic lupus erythematosus show high levels of soluble FAS.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human FAS
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Blocking - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking activity. The ND50 is 0.01– 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2716048 (BioLegend Cat. No. 684401) AB_2716055 (BioLegend Cat. No. 684402)
Structure: Trimer
Distribution: Activated human T cells, B cells, malignant human lymphoid cell lines, fibroblasts, dendritic cells, thymocytes, macrophages, cardiomyocytes, hepatocytes, FAS is expressed on most cell types.
Function: Apoptotic clearance of virus-infected cells, contributes to shutdown of chronic immune responses and to maintenance of peripheral tolerance. Soluble FAS modulates cell apoptosis.
Interaction: Lymphocytes T (mainly CD8+), NK cells.
Ligand/Receptor: FASL (TNFSF6)
Cell Type: T cells, B cells, Fibroblasts, Dendritic cells, Thymocytes, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Oehm A, et al. 1992. J Biol Chem. 267:10709. 2. Cheng J, et al. 1994. Science. 263:1759. 3. Papoff G, et al. 1996. J Immunol. 156:4622. 4. Rescigno M, et al. 2000. J Exp Med. 192:1661. 5. Giroux M, et al. 2005. Blood. 105:703. 6. Kovacic N, et al. 2010. Expert Opin Ther Targets 14:1121.
Gene ID: 355
UniProt: View information about CD95 on UniProt.org
Clone: A16086F
Regulatory Status: RUO
Other Names: Tumor necrosis factor receptor superfamily member 6 (TNFRSF6), ALPS1A, APO-1, Apoptosis antigen 1 (APT1), CD95, FAS1, FASTM, FS-7-associated surface antigen
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.