Biolegend, 685502, Purified anti-HDAC8 Antibody, 100μg

Histone deacetylases (HDACs) are a family of enzymes responsible for the removal of acetyl groups from lysine residues on the core histones and other non-histone proteins. HDAC8 is a type I histone deacetylase and was initially found to deacetylate a variety of acetylated histone variants in vitro . HDAC8 is a kinectically and structurally well-characterized deacetylase that was reported to catalyze several nuclear proteins, which include ERR-α, hEST1B, and SMC3. Patients with HDAC8 mutations show Cornelia de Lange Syndrome (CdLS) and Wilson-turner Syndrome (WTS) phenotypes. Elevated expression of HDAC8 has been observed in various tumor types, such as acute lymphoblastic leukemia (AML), neuroblastomas, and glimoas. HDAC8 is implicated as a therapeutic target in cancer and infectious diseases.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human HDAC8 recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Aru B, et al. 2020. ACS Omega. 5:25854. PubMed
RRID: AB_2572204 (BioLegend Cat. No. 685502)
Structure: 377 amino acids with a predicted molecular weight of 41.8 kD. It belongs to the type I Histone deacetylase subfamily.
Distribution: Nucleus and cytoplasm.
Function: HDAC8 is a deacetylase and regulates gene expression through catalyzing the removal of acetyl groups on lysine residues in the core histones and transcriptional factors.
Interaction: Interacts with PEPB2-MYH11, CBFA2T3, SMG5, and alpha-SMA.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Qi J, et al. 2015. Cell Stem Cell. 17:597. 2. Tian Y, et al. 2015. Cancer Res. 75:4803. 3. Feng L, et al. 2015. J. Hum. Genet. 60:165. 4. Ha SD, et al. 2014. J. Immunol. 193:1333. 5. Higuchi T, et al. 2013. Blood 121:3640. 6. Deardorff MA, et al. 2012. Nature 489:313.
Gene ID: 55869
UniProt: View information about HDAC8 on UniProt.org
Clone: W15090A
Regulatory Status: RUO
Other Names: Histone deacetylase-like 1 (HDACL1), MRXS6, Wilson-Turner X-linked mental retardation syndrome (WTS), RPD3
Isotype: Mouse IgG1, κ