Biolegend, 686004, PE anti-STAT6 Phospho (Tyr641) Antibody, 100tests

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human STAT6 peptide phosphorylated at Tyr 641
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Human STAT6 has three isoforms; the molecular weights are 94, 82 and 74kD. The immunogen (phosphorylated peptide) is shared by these three isoforms. This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms. The predominant band detected is at 94 kD.Clone A15137E does not react with mouse.This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: Takeuchi I, et al. 2023. J Allergy Clin Immunol. 1402:151. PubMed Fang F, et al. 2022. JCI Insight. 7:. PubMed
RRID: AB_2629546 (BioLegend Cat. No. 686003) AB_2629547 (BioLegend Cat. No. 686004)
Structure: 847 amino acids with a predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution: Cytoplasm; translocates to the nucleus when phosphorylated.
Function: STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling. While STAT6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following phosphorylation by Janus kinases, STAT6 translocates to the nucleus where it regulates gene transcription.
Interaction: STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: 1. Goenka S, et al. 2011. Immunol. Res. 50:87. 2. Wurster AL, et al. 2000. Oncogene 19:2577. 3. Akira S. 1999. Stem Cells 17:138. 4. Zamorano J, et al. 2005. J. Immunol. 174:2843. 5. David M, et al. 2001. Oncogene 20:6660. 6. Takeda K, et al. 1996. Nature 380:627.
Gene ID: 6778
UniProt: View information about STAT6 Phospho Tyr641 on UniProt.org
Clone: A15137E
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 6, IL-4 Stat
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.