Biolegend, 686401, Purified anti-STAT1 Phospho (Ser727) Antibody, 25μg

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human STAT1 peptide phosphorylated at Ser 727.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC, ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Morisaki Y, et al. 2023. Front Cell Neurosci. 1308972:17. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Wu Y, et al. 2021. J Clin Invest. 131:. PubMed Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
RRID: AB_2861070 (BioLegend Cat. No. 686401) AB_2616883 (BioLegend Cat. No. 686402)
Structure: 750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution: Translocates to the nucleus when phosphorylated.
Function: Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction: Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: 1. Durbin JE, et al. 1996. Cell. 84:443. 2. Darnell JE Jr, et al. 1994. Science 264:1415. 3. Chen X, et al. 1998. Cell. 93:827. 4. Ramana CV, et al. 2000. Oncogene. 19:2619.
Gene ID: 6772
UniProt: View information about STAT1 Phospho Ser727 on UniProt.org
Clone: A15158B
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype: Mouse IgG1, κ