Biolegend, 686702, Purified anti-CCL7 (MCP-3) Antibody, 100μg

CCL7, also known as MCP-3, was initially purified from cytokine-stimulated human osteosarcoma cell line (MG-63) conditioned medium. CCL7 has high similarity with CCL2/MCP-1 (71%), CCL8/MCP-2 (58%), and CCL13/MCP-4 (59%) at the amino acid level. Chemokines play a key role in inflammation and, in the initial phase, CXC chemokines chemoattract polymorphonuclear leukocytes (PMNs). The next step in the inflammatory processes is directed in part by CC chemokines, including the monocyte chemoattractant proteins (CCL2, CCL7, CCL8, and CCL13) that chemoattract additional leukocytes subsets. It has been reported that matrix metalloproteinases (MMPs) regulate chemokine bioactivity, increasing or decreasing their chemoattractant property. MMP-8 and MMP-9 modulate CXC chemokines, and MMP-2 truncates the N-terminal of CCL7, eliminating the first four amino acids producing CCL7 (5-76 residues), which leads to a loss of activity. The truncated CCL7 binds to its receptor, but it does not induce signaling transduction. Monocyte chemoattractant proteins (MCPs) are truncated by different MMPs to create potent antagonists and induce decreases in inflammation. For example, MMP-13 cleaves CCL2 and CCL7 and generates potent receptor antagonists. Also, CCL7 and CCL2 play an important role in the pathogenesis of systemic sclerosis (SSc). It has been described that they are overexpressed in SSc and activate collagen production in dermal fibroblasts. In addition, CCL7 and CCL21 are highly expressed in gastric cancer and are associated with lymph node metastasis and poor prognosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant human CCL7.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2616886 (BioLegend Cat. No. 686702)
Structure: Chemokine.
Distribution: Fibroblasts, mast cells, astrocytes, endothelial cells, monocytes, and macrophages.
Function: CCL7 chemoattracts monocytes, T cells, NK cells, eosinophils, and basophils. CCL7 protein is secreted by mononuclear leukocytes after stimulation with IL-1β, IFN-α, IFN-β, measles virus, poly I-C, or Con A. In fibroblasts, CCL7 mRNA and protein are synergistically induced after treatment with IL-1β and IFN-γ. CCL7 is stimulated in murine fibroblasts and astrocytes by TGF-β and TNF-α respectively. Anti-inflammatory cytokines (IL-4, IL-10, or IL-13) inhibit the expression of CCL7 in monocytes.
Interaction: Monocytes, T cells, NK cells, dendritic cells, mast cells, eosinophils, and basophils.
Ligand/Receptor: CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195).
Cell Type: Fibroblasts, Mast cells, Astrocytes, Endothelial cells, Monocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.2. Menten P, et al. 1999. Eur. J. Immunol. 29:678.3. McQuibban GA, et al. 2000. Science 289:1202.4. Menten P, et al. 2001. Eur. Cytokine Netw. 12:554.5. Dean RA, et al. 2008. Blood 112:3455.6. Renner NA, et al. 2011. J. Neurovirol. 17:146.7. Hwang TL, et al. 2012. World J. Gastroenterol. 18:1249.8. Feuser K, et al. 2012. Cytokine 58:178.
Gene ID: 6354
UniProt: View information about CCL7 on UniProt.org
Clone: A15027D
Regulatory Status: RUO
Other Names: Monocyte chemotactic protein3 (MCP-3), Small inducible cytokine subfamily A, member 7 (SCYA7)
Isotype: Mouse IgG1, κ