Biolegend, 687002, Purified anti-ERK1 Antibody, 100μg

ERK1 is a member of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1 and ERK2 share 85% homology and both are involved in the MAP kinase signaling cascade. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Product was tested in IHC using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween 20
RRID: AB_2616892 (BioLegend Cat. No. 687002)
Structure: 379 amino acids with a predicted molecular weight of 43.1 kD. Contains a kinase domain responsible for phosphorylation of down stream substrates.
Distribution: Nucleus and cytoplasm.
Function: ERK1 is a serine/threonine kinase which acts as a key component of the MAP kinase signaling pathway. ERK1 and ERK2 have been shown to play redundent roles in cell proliferation, differentiation, migration, survival, and apoptosis.
Interaction: Interacts with ADAM15, ARRB2, CANX, DAPK1, HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, SGK1, TPR, CDKN2AIP, PEA15, and MKNK2.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
Gene ID: 5595
UniProt: View information about ERK1 on UniProt.org
Clone: W15133A
Regulatory Status: RUO
Other Names: Extracellular signal-regulated kinase 1, Mitogen-activated protein kinase 3 (MAPK3), p44ERK1, p44-MAPK, PRKM3
Isotype: Rat IgG2a, κ