Biolegend, 687202, Purified anti-TP63 Antibody, 100μg

TP63 is a p53-related gene and shares similar domain organization with p53 and p73. Full length TP63 contains a N-terminal transactivation domain, a central DNA binding domain, and a C-terminal oligomerization domain responsible for the formation of a homotetramer. TP63 have several isoforms produced by alternative splicing. Most of the isoforms contain the DNA binding domain, but differ in their transactivation and oligomerization domains. The transactivation domain is present in the transcriptional active isoforms (TA-p63) and is absent in the truncated isoforms (ΔN -p63). TP63 is expressed in keratinocyte stem cells and is essential for the morphogenesis and development of ectodermal, orofacial, and limb tissues. TP63 deficient mice die soon after birth with skin and limb developmental defects.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human TP63 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL (1:250-1:1000 dilution). For ChIP applications, the suggested dilution is 1:50 - 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Application Notes: This clone predominantly recognizes ?N-p63, and weakly crosses react with TA-p63. Both ?N-p63 and TA-p63 have multiple isoforms, the largest isoforms are 66kD and 77kD respectively. This antibody recognizes more than one isoform of ?N-p63.
Product Citations: Vaidyanathan S, et al. 2022. Mol Ther. 30:223. PubMed Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed Mohibi S, et al. 2020. J Invest Dermatol. 140:2166. PubMed Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
RRID: AB_2616941 (BioLegend Cat. No. 687202)
Structure: p53 family of transcription factors. 12 reported isoforms produced by alternative splicing. Predicted molecular weight ranged from 44.7kD to 76.8kD.
Distribution: Nuclear.
Function: TP63 acts as a transcriptional activator or repressor, is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration.
Interaction: Forms a homotetramer. Interacts with HIPK2, SSRP1, WWP1, PDS5A, and NOC2L.
Biology Area: Cell Biology, Transcription Factors
Antigen References: 1. Katoh I, et al. 2016. Cell Cycle 15:699. 2. Candi E, et al. 2014. Hum. Mutat. 35:702. 3. Chari NS, et al. 2013. Cell Death Differ. 20:1080. 4. Vasmatzis G, et al. 2012. Blood 120:2280. 5. Scott DW, et al. 2012. Blood 119:4949. 6. Miki D, et al. 2010. Nat. Genet. 42:893.
Gene ID: 8626
UniProt: View information about TP63 on UniProt.org
Clone: W15093A
Regulatory Status: RUO
Other Names: Tumor protein p63, p63, Chronic ulcerative stomatitis protein (CUSP), Keratinocyte transcription factor (KET), Tumor protein p73-like (p73L), TP63
Isotype: Mouse IgG2b, κ