Biolegend, 687502, Purified anti-DNMT1 Antibody, 100μg

DNA (Cytisube-5-)-Methyltransferase 1 (DNMT-1), is an enzyme that has a role in the establishment and regulation of tissue-specific patterns of  methylated cytosine residues. This enzyme associates with DNA replication sites in the S phase and methylates CpG residues to maintain the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. In addition to this, it associates with DNA during the G2 and M phases to maintain DNA methylation. Diseases associated with abnormal DNMT-1 function include certain human tumors and developmental abnormalities. There are two transcript encoding different isoforms have been found for this gene.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human DNMT1 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2616944 (BioLegend Cat. No. 687502)
Structure: 1616 amino acids with a predicted molecular weight of 183.2 kD. Contains a C-terminal catalytic domain responsible for DNA methylation.
Distribution: Nuclear.
Function: DNMT1 is a DNA methyltransferase that is responsible for the establishment and maintaining DNA methylation patterns.
Interaction: Forms a homodimer. Interacts with CSNK1D, HDAC1, PCNA, DMAP1, HDAC2, MBD2, MBD3, UBC9, UHRF1, and USP7.
Biology Area: Cell Biology, Neuroscience, Transcription Factors
Antigen References: 1. Merry CR, et al. 2015. Hum. Mol. Genet. 24:6240.2. Cheng J, et al. 2015. Nat. Commun. 6:7023.3. Pathania R, et al. 2015. Nat. Commun. 6:6910.4. Liao J, et al. 2015. Nat. Genet. 47:469.5. Espada J. 2012. Epigenetics 7:115.6. Svedružić ŽM. 2011. Prog. Mol. Biol. Transl. Sci. 101:221.
Gene ID: 1786
UniProt: View information about DNMT1 on UniProt.org
Clone: W15077A
Regulatory Status: RUO
Other Names: DNA (cytosine-5-)-methyltransferase 1, CXXC9, MCMT, AIM, DNMT, HSN1E, ADACADN, m. Hasl
Isotype: Mouse IgG1, κ