Biolegend, 688802, Purified anti-CXCL12 (SDF-1β) Antibody, 100μg

CXCL12, also known as SDF-1, has different variants, which include SDF-1α, SDF-1β, and SDF-1γ derived from alternative splicing. SDF-1 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. The SDF-1/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Data suggests that SDF-1β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial mouse CXCL12 (SDF-1β) recombinant protein (22-93 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629642 (BioLegend Cat. No. 688802)
Structure: 72 amino acids with a predicted molecular weight of approximately 8.5 kD and observed molecular weight of approximately 12 kD.
Distribution: Expressed by bone marrow stromal cells, endothelial cells, epithelial cells, pericytes, dendritic cells, and fibroblasts.
Function: SDF-1 is a constitutively produced chemokine involved in lung, brain, joint, and intestine inflammation. SDF-1 is chemotactic for T, B, and cancer sells. SDF-1/CXCR4 pair plays a role in the mobilization of CD34+ progenitors to peripheral blood. SDF-1 is important in morphogenesis, angiogenesis, and cancer metastasis.
Interaction: CXCR4 and ACKR3.
Ligand/Receptor: CXCR4.
Cell Type: Endothelial cells, Epithelial cells, Dendritic cells, Fibroblasts
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Nagasawa T, et al. 1994. Proc. Natl. Acad. Sci. USA 91:2305.2. Muller A, et al. 2001. Nature 410:50.3. Valenzuela-Fernandez A, et al. 2002. J. Biol. Chem. 277:15677.4. De La Luz Sierra M, et al. 2004. Blood 103:2452.5. Kortesidis A, et al. 2005. Blood 105:3793.6. Davis DA, et al. 2005. Blood 105:4561.7. Zlotnik A. 2008. J. Pathol. 215:211.8. Herberg S, et al. 2013. PLoS One 8:e58207.
Gene ID: 6387
UniProt: View information about CXCL12 on UniProt.org
Clone: W15149A
Regulatory Status: RUO
Other Names: Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1β), TLSF, TPAR1, SYC12, IRH
Isotype: Rat IgG2a, κ