Biolegend, 688902, Purified anti-GSK-3α Antibody, 100μg

Glycogen Synthase Kinase 3 Alpha (GSK-3α) is a serine/threonine protein kinase which mediates the control of several regulatory proteins involved in glucose homeostasis, including glycogen synthase, and transcription factors such as JUN. It also plays a role in the WNT and PI3K signaling pathways. GSK-3α contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting glycogen synthase (GYS1 or GYS2) activity and mediates the development of insulin resistance. In Wnt signaling, GSK-3α regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. GSK-3α is also reported to facilitate the generation of amyloid precursor protein (APP)-derived amyloid plaques found in Alzheimer disease. To date, there are six GSK-3α mutant mouse lines generated, these stains express various abnormal phenotypes in growth, size, mortality, aging, adipose morphology, homeostasis and metabolism, muscle and the nervous system.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial human GSK-3α recombinant protein (251-483 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2629652 (BioLegend Cat. No. 688902)
Structure: 483 amino acids with a predicted molecular weight of 51 kD. GSK-3α is a serine/threoenine kinase.
Distribution: Cytoplasm.
Function: GSK-3α is a serine/threonine kinase, mediating signals in the WNT and the PI3K pathways. GSK-3α is implicated in the regulation of glucose metabolism and homeostasis.
Interaction: Interacts with ARRB2, AXIN1, CTNNB1, and CTNND2.
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Park SA, et al. 2016. PLoS One 11:e0153075. 2. Ye C, et al. 2015. J. Neurochem. 133:273. 3. Grassilli E, et al. 2014. PLoS One 9:e100947. 4. Nekova TS, et al. 2014. Br. J. Haematol. 167:272. 5. Zhou J, et al. 2013. J. Clin. Invest. 123:1821. 6. Jaworski T, et al. 2011. Nature 480:e4.
Gene ID: 2931
UniProt: View information about GSK-3alpha on UniProt.org
Clone: W15183A
Regulatory Status: RUO
Other Names: Glycogen synthase kinase alpha, GSK-3 alpha
Isotype: Rat IgG2a, κ