Biolegend, 689202, HRP Protein A, 500microl

Protein A is a 42 kD surface protein originally found in the cell wall of the bacterium Staphylococcus aureus . It is widely used in biochemical research and industry because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs. It binds the heavy chain within the Fc region of most immunoglobulins. HRP Protein A is useful for detecting primary antibodies, e.g. , in ELISA, ELISPOT, IHC, or Western blotting. For IP followed by Western blotting application, HRP protein A will avoid IgG heavy/light chain issue because it only recognizes the IgG in its native form, and the antibody used to IP the target is usually boiled with the immunoprecipitates and gets denatured.
500microl
Verified Reactivity: Mouse, Rabbit
Formulation: This reagent is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: Recombinant protein A was conjugated with HRP under optimal conditions.
Concentration: 1.0 mg/ml
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: For Western blot analysis, the reagent should be titrated between 1:2000 - 1:8000 to determine optimal conditions. Affinity of Protein A to Ig Types from Different Species: Species Immunoglobulin Binding to Protein A Mouse IgG1 + IgG2a ++++ IgG2b +++ IgG3 ++ IgM - Rat IgG1 - IgG2a - IgG2b - IgG2c ++ Human Total IgG ++++ IgG1 ++++ IgG2 ++++ IgG3 - IgG4 ++++ IgM - IgA - IgE - Chicken IgY - Goat IgG +/- Hamster IgG + Rabbit IgG ++++ Sheep IgG +/-
Product Citations: Bauer A, et al. 2023. Pharmaceutics. :15. PubMed
Structure: 42 kD surface protein.
Function: Binds IgG.
Biology Area: Cell Biology
Antigen References: 1. Verwey WF. 1940. J. Exp. Med. 71:635. 2. Graille M, et al. 2000. Proc. Natl. Acad. Sci. USA 97:5399.
Gene ID: NA
UniProt: View information about Protein A on UniProt.org
Regulatory Status: RUO
Other Names: Protein A