Biolegend, 690452, Purified anti-STAT3 Phospho (Tyr705) Recombinant Antibody, 100μg

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to STAT3 phosphorylated at tyrosine 705
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone shows high pan-reactivity to STAT3 when used for WB. BioLegend clone 13A3-1 is more suitable for phospho-specific detection of STAT3. This recombinant antibody clone is derived from clone A16002B.
RRID: AB_2894516 (BioLegend Cat. No. 690451) AB_2894516 (BioLegend Cat. No. 690452)
Structure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution: Ubiquitous
Function: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases. The small GTPase Rac1 binds and regulates activity of STAT3.
Ligand/Receptor: When activated, STAT3 dimerizes and translocates to the nucleus where it regulates gene expression
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Akira S, et al. 1994. Cell. 77:63. Zhang X, et al. 1995. Science. 267:1990. Sanchez-Margalet V, et al. 2001. Cell Immunol. 211:30. Simon A, et al. 2000. Science. 290:144. Hoey T, et al. 1999. Adv Immunol. 71:145.
Gene ID: 6774
UniProt: View information about STAT3 on UniProt.org
Clone: A16002B.Rec
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype: Mouse IgG1, κ