Biolegend, 690902, Purified anti-NOS2 Antibody, 100μg

Nitric Oxide Synthase 2 (NOS2), also known as inducible NOS (iNOS), contains an N-terminal oxygenase domain and a C-terminal reductase domain, and functions to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a reactive free radical which acts as a biologic messenger with diverse functions throughout the body, such as neurotransmission, antimicrobial, and antitumor activity. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. NOS2 also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such as COX2.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NOS2 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Li T, et al. 2023. Adv Sci (Weinh). 10:e2206517. PubMed McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
RRID: AB_2629826 (BioLegend Cat. No. 690902)
Structure: 1153 amino acids with a predicted molecular weight of 131 kD. Contains a flavodoxin-like domain and a FAD-binding FR-type domain.
Distribution: Cytoplasm.
Function: NOS2 is a nitric oxide synthase and produces large quantity of NO in response to the stimulation of proinflammatory cytokines. NOS2 plays an important role in host defense against pathogens and participates in anti-tumor processes.
Interaction: Forms a homodimer. Interacts with SLC9A3R1, GAPDH, S100A8, and S100A9.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Salim T, et al. 2016. PLoS One. 11:e0153289. 2. Ma Z, et al. 2016. Inflammation. 39:543. 3. Lee SH, et al. 2016. J. Cell. Biochem. 117:978. 4. Landes MB, et al. 2015. J. Leuko. Biol. 97:1111. 5. Karpuzoglu E, et al. 2006. Nitric. Oxide 15:177. 6. Suschek CV, et al. 2004. Curr. Mol. Med. 4:763.
Gene ID: 4843
UniProt: View information about Nos2 on UniProt.org
Clone: 5C1B52
Regulatory Status: RUO
Other Names: Nitric oxide synthase 2, nitric oxide synthase 2A, NOS2A, HEP-NOS, inducible nitric oxide synthase (iNOS)
Isotype: Mouse IgG1, κ