Biolegend, 691803, PE anti-RPS6 Antibody, 25tests

The eukaryotic ribosome is composed of 40S and 60S subunits, which is involved in the physiological process of protein translation. In many organisms, ribosomal proteins undergo various post-translational modifications, including phosphorylation, acetylation, methylation, O-linked b-N-acetylglucosaminylation, and ubiquitylation. Ribosomal proteinS6 (RPS6), the first identified ribosomal protein which can be phosphorylated, is a component of eukaryotic ribosomal 40S subunit. Functional studies of RPS6 phosphorylation suggested its role in the regulation of protein synthesis, cell proliferation, and glucose homeostasis. The phosphorylation of RPS6 can be triggered by multiple stimuli including growth factors, tumor promoting agents, and mitogens. The inhibition of RPS6 phosphorylation was found mainly under stress conditions such as hypoxia, heat shock, and hyperosmolarity. RPS6 phosphorylation is also commonly used as a marker for neuronal activity and a read out of mammalian target of rapamycin complex 1 (mTORC1) activity.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human S6 peptide.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2734535 (BioLegend Cat. No. 691803) AB_2734536 (BioLegend Cat. No. 691804)
Structure: 249 amino acids with a predicted molecular weight of 28.7kD.
Distribution: Cytoplasm and nucleus.
Function: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Interaction: NCBP1, PASK.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673. 2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75. 3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350. 4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223. 5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199. 6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1. 7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41.
Gene ID: 6194
UniProt: View information about RPS6 on UniProt.org
Clone: A16009C
Regulatory Status: RUO
Other Names: 40S ribosomal protein S6, Ribosomal Protein S6
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.