Biolegend, 692151, Purified anti-RSK1 Phospho (Ser380) Antibody, 25μg

RSK1 (ribosomal S6 kinase 1) is a serine/threonine kinase that plays a pivotal role in various cellular processes, including cell growth, survival, and proliferation. It is activated by the ERK/MAPK signaling pathway and subsequently phosphorylates a range of substrates involved in transcription, translation, and cell cycle regulation. RSK1 is particularly important in cancer biology, where its aberrant activation is linked to the progression of several malignancies, including breast, prostate, and lung cancers. Additionally, RSK1 is implicated in cardiovascular diseases and inflammatory responses. Phosphorylation at serine 380 is crucial for RSK1 full activation. This modification creates a docking site for PDK1, which further phosphorylates RSK1, leading to its complete activation and enabling it to phosphorylate downstream targets. Dysregulation of this phosphorylation event can disrupt normal cellular functions and contribute to disease pathogenesis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human RSK1 phosphorylated at Ser380
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.031 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: This antibody is recommended for intracellular staining for flow cytometry (ICFC) using Cyto-Fast™ Fix/Perm Buffer Set (Cat No. 426803), or True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3683500 (BioLegend Cat. No. 692151) AB_3683500 (BioLegend Cat. No. 692152)
Structure: The RSK1 protein consists of 735 amino acids with a molecular weight of approximately 83 kDa.
Distribution: RSK1 is primarily located in the cytoplasm and nucleus of the cell. Upon activation translocates from the cytoplasm to the nucleus.
Function: • Cell Growth and Proliferation: By phosphorylating and activating transcription factors such as c-Fos and serum response factor (SRF), RSK1 promotes cell growth and proliferation. • Cell Survival: RSK1 enhances cell survival by phosphorylating and inhibiting proapoptotic proteins like BAD, thereby preventing apoptosis. • Protein Synthesis: It also phosphorylates ribosomal protein S6, which is involved in the initiation of protein synthesis.
Interaction: • ERK1/2: RSK1 is activated by ERK1/2 through phosphorylation, which is a critical step in the ERK/MAPK signaling pathway. • PDK1: Phosphorylation by PDK1 at serine 380 is essential for the full activation of RSK1. • CREB-binding protein (CBP): RSK1 interacts with CBP, a transcriptional coactivator, to regulate gene expression. • Fibronectin1: RSK1 modulates the expression of Fibronectin1, affecting cell adhesion. • CDK2: RSK1 influences the expression of CDK2, impacting cell cycle progression and DNA replication. • mTORC1 components: RSK1 phosphorylates and regulates components of the mTORC1 complex, such as Raptor, promoting cell growth and metabolism
Biology Area: Cancer Biomarkers, Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Neuroscience
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Wu W, et al. 2024. Breast Cancer Research. 26:146. Lara R, et al. 2013. Cancer Res. 73:5301-8. Mao S, et al. 2022. PLoS Biology. 3001653. Anjum R, et al. 2008. Nature Reviews Molecular Cell Biology. 9:747-58.
Regulation: • Phosphorylation: Activation of RSK1 involves a series of phosphorylation events. ERK phosphorylates RSK1, leading to its activation. A critical phosphorylation site is serine 380, which creates a docking site for PDK1, further phosphorylating and fully activating RSK1. • Subcellular Localization: RSK1 shuttles between the cytoplasm and the nucleus, allowing it to phosphorylate different substrates depending on its location. Feedback Mechanisms: RSK1 activity is also regulated by feedback loops within the ERK/MAPK pathway, ensuring balanced cellular responses. • Feedback Mechanisms: RSK1 activity is also regulated by feedback loops within the ERK/MAPK pathway, ensuring balanced cellular responses.
Gene ID: 6195
UniProt: View information about RSK1 Phospho Ser380 on UniProt.org
Clone: A23014F
Regulatory Status: RUO
Other Names: MAPKAPK1, P90Rsk, MAP Kinase-Activated Protein Kinase 1a, 90 KDa Ribosomal Protein S6 Kinase 1, Ribosomal Protein S6 Kinase Alpha-1, MAPKAP Kinase 1a
Isotype: Mouse IgG2b, κ