Biolegend, 694702, Purified anti-human IL-32α Antibody, 100μg

IL-32 is a proinflammatory cytokine with both extracellular and intracellular functions. IL-32 was originally identified as a natural killer transcript 4 (NK4). Several isoforms of IL-32 have been identified, and IL-32α is the most abundant. Overexpression of IL-32α in myeloid cell lines enhances natural killer cell-mediated killing, and IL-32 expression can be induced by IL-18 in NK cells. IL-32α can be released by some epithelial cell lines in response to INF-γ, TNF-α, and IL-1β. IL-32 can induce production of various cytokines including TNF-α, IL-1β, IL-8 and IL-6 through NF-κB and p38 MAPK activation. IL-32 works synergistically with IL-17 to induce osteoclast differentiation. Recombinant IL-32α can induce macrophage apoptosis in the presence of M. tuberculosis. Intracellular IL-32α increases IL-6 production by interacting with PKCε and STAT3, which not only increases STAT3 phosphorylation but also enhances STAT3 recruitment to the IL-6 promoter. IL-32 can synergize with NOD1 and NOD2 ligands to induce IL-1β and IL-6 through the caspase I-dependent pathway. IL-32 has been associated with pathogenesis of many chronic inflammatory diseases including rheumatoid arthritis and ulcerative colitis. IL-32 is highly expressed in various cancers including gastric and pancreatic cancer. Studies have suggested that IL-32 can function as an angiogenic factor. In addition, IL-32α expression in liver cells is increased following progression of many liver diseases. Also, IL-32 is involved in the immune response to many viruses including HIV, influenza A, hepatitis B, and papillomavirus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-32α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A15159A does not cross-react with mouse (in-house tested).
RRID: AB_2650733 (BioLegend Cat. No. 694702)
Structure: 131 amino acids with a predicted molecular weight of 15 kD.
Distribution: Secreted by T cells, natural killer cells, dendritic cells, and keratinocytes.
Function: IL-32α expression can be induced by IL-18, IL-1β, IFN-γ, IL-12 and IL-17. The activity of IL-32α can be significantly increased by limited cleavage of IL-32α by neutrophil proteinase 3.
Interaction: IL-32α has been reported to interact with neutrophil proteinase 3, PKC, STAT3, integrin and paxilin.
Cell Type: T cells, NK cells, Dendritic cells
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Joosten LA, et al. 2013. Cell. Mol. Life Sci. 70:3883.2. Kang JW, et al. 2012. J. Biol. Chem. 287:35556.3. Cheon S, et al. 2011. J. Biol. Chem. 286:12049.4. Nishida A, et al. 2009. J. Biol. Chem. 284:17868.5. Heinhuis B, et al. 2012. J. Biol. Chem. 287:5733.6. Novick D, et al. 2006. Proc. Natl. Acad. Sci. USA 103:3316.7. Shioya M, et al. 2007. Clin. Exp. Immunol. 149:480.
Gene ID: 9235
UniProt: View information about IL-32alpha on UniProt.org
Clone: A15159A
Regulatory Status: RUO
Other Names: IL-32 alpha, Natural killer cells protein 4 (NK4), Natural killer transcript 4 (NK4), Tumor necrosis factor alpha-inducing factor (TAIF)
Isotype: Mouse IgG2b, κ