Biolegend, 695302, Purified anti-EOS Antibody, 100μg

EOS, also known as IKZF4 (Ikaros family zinc finger 4), is a member of the Ikaros family of zinc finger transcription factors, which are key transcriptional repressors during development of lymphocytes. Each of the five Ikaros family members (Ikaros, Helios, Aiolos, Eos and Pegasus) has N-terminal and C-terminal zinc-finger motifs responsible for DNA binding and dimerization, respectively. EOS is a highly conserved DNA-binding protein that recognizes the typical 5'WGGAAT-3' Ikaros-binding sequence. EOS has been implicated in the development of lymphoid cells and nervous systems, as well as mediating FOXP3-dependent gene silencing in CD4 + regulatory T cells though interacting with FOXP3 and CTBP1.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human EOS recombinant protein (271-520 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:1000 - 1:250 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W15169A does not cross-react with mouse (in-house tested). This antibody recognizes both isoforms of EOS and it also detects a non-specific protein of approximately 75 kD in the K562 cell line tested.
RRID: AB_2715762 (BioLegend Cat. No. 695302)
Structure: EOS has two isoforms. Isoform 1 has 585 amino acids with a predicted molecular weight of 64.1 kD. Isoform 2 has 544 amino acids with a predicted molecular weight of 59.7 kD. Both isoforms contain six zinc finger domains responsible for DNA-binding or prot
Distribution: Nucleus
Function: EOS is a zinc finger transcription factor that is implicated in the development of lymphoid cells and nervous systems.
Interaction: EOS forms homodimers or heterodimers with other Ikaros family members. Interacts with CTBP2, SPI1, MITF, FOXP3, and CTBP1.
Antigen References: 1. Rieder SA, et al. 2015. J. Immunol. 195:553. 2. Raffin C, et al. 2013. J. Immunol. 191:4619. 3. Paradzik M, et al. 2012. Int. J. Immunopathol. Pharmacol. 25:893. 4. Bettini ML, et al. 2012. Immunity. 36:717. 5. John LB, Ward AC. 2011. Mol. Immunol. 48:1272. 6. Pan F, et al. 2009. Science. 325:1142.
Gene ID: 64375
UniProt: View information about EOS on UniProt.org
Clone: W15169A
Regulatory Status: RUO
Other Names: Ikaros family zinc finger protein 4 (IKZF4), KIAA1782, ZNFN1A4
Isotype: Mouse IgG1, κ