Biolegend, 695502, Purified anti-BAD Antibody, 100μg

BCL-2 associated agonist of cell death (BAD) is a member of the BCL-2 family, which is involved in the regulation of the mitochondrial apoptosis pathway. The BCL-2 protein family members can either promote cell survival (BCL-2, BCL-XL, and MCL-1) or induce apoptosis (BAX, BAK, BIM, BID, and BAD). The BCL-2 family proteins form homodimers or heterodimers through the conserved BCL-2 homology motifs (BH1, BH2, BH3, and BH4). BAD belongs to the BH3-only proteins and functions as a direct antagonist of the prosurvival effect mediated by BCL-2 and BCL-XL. BAD is inactivated by several survival kinases, such as AKT, PKA, and RSK, through phosphorylation. Upon stimulation of survival signals, phosphorylated BAD dissociates from BCL-2/ BCL-XL complexes and is sequestered in the cytoplasm by 14-3-3 scaffold proteins. Without interacting with BAD, BCL-2 and BCL-XL are able to suppress apoptosis by preventing release of cytochrome c from mitochondria and blocking subsequent activation of caspases.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length mouse BAD recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Chea C, et al. 2018. PLoS One. 13:e0191683. PubMed
RRID: AB_2650854 (BioLegend Cat. No. 695502)
Structure: 204 amino acids with a predicted molecular weight of 22.1 kD. Contains a BH3 domain responsible for dimer formation.
Distribution: Mitochondrion outer membrane and cytoplasm.
Function: BAD is a proapoptotic protein, promoting cell apoptosis through inactivating the BCL-2 family members BCL-2 and BCL-XL.
Interaction: Forms homodimers or heterodimers with other members of the same family. Interacts with S100A10, 14-3-3, AKT1, PIM3, NOL3, and HIF3A.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Antigen References: 1. Cekanova M, et al. 2015. Exp. Cell. Res. 331:1. 2. Pareja F, et al. 2014. Mol. Cancer Res. 12:987. 3. Sakamaki J, et al. 2011. Proc. Natl. Acad. Sci. USA 108:6085. 4. Yu C, et al. 2004. Mol. Cell. 13:329-40. 5. Cartier A, et al. 2003. Exp. Cell Res. 291:242. 6. Moriishi K, et al. 2002. Virology 292:258.
Gene ID: 12015
UniProt: View information about BAD on UniProt.org
Clone: W16055A
Regulatory Status: RUO
Other Names: Bcl2-associated agonist of cell death, Bcl-2-binding component 6 (Bbc6), Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death.
Isotype: Rat IgG2a, κ