Biolegend, 695603, PE anti-BACH2 Antibody, 25tests

BTB and CNC Homolog 2 (BACH2) is a transcription factor of the basic leucine zipper family. BACH2 participates in the regulation of both innate and adaptive immune responses. In B cells, BACH2 plays critical roles in somatic hypermutation, class-switch recombination, memory B cell formation, and repression of myeloid genes. In T cells, BACH2 promotes the development of Foxp3+ regulatory T cells and memory T cells. Bach2 is required for the maintenance of naive CD4 T cells by suppressing effector T cell differentiation. BACH2 is also required for the maturation and function of alveolar macrophages. Defects in BACH2 gene are associated with autoimmune and allergic diseases, such as asthma, type 1 diabetes, celiac disease, rheumatoid arthritis, and generalized vitiligo.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human BACH2 recombinant protein (513-841 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2922645 (BioLegend Cat. No. 695603) AB_2922645 (BioLegend Cat. No. 695604)
Structure: 841 amino acids with a predicted molecular weight of 92.5 kD. Contains a N-terminal BTB/POZ domain for protein-protein interaction and a C-terminal bZIP domain for DNA binding.
Distribution: Nucleus and cytoplasm.
Function: BACH2 is a transcription factor involved in the development and differentiation of both B and T cells.
Interaction: Forms homodimers or heterodimers with Maf-related transcription factors.
Cell Type: B cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kuwahara M, et al. 2016. Nat. Commun. 7:12596. 2. Sidwell T, et al. 2016. Nat. Immunol. 17:744. 3. Roychoudhuri R, et al. 2016. Nat. Immunol. 17:851. 4. Itoh-Nakadai A, et al. 2014. Nat. Immunol. 15:1171. 5. Kuwahara M, et al. 2014. Nat. Commun. 5:3555. 6. Kim EH, et al. 2014. J. Immunol. 192:985.
Gene ID: 60468
UniProt: View information about BACH2 on UniProt.org
Clone: 16B10B53
Regulatory Status: RUO
Other Names: BTB and CNC homolog 2, Transcription regulator protein BACH2,
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.