Biolegend, 696006, Purified anti-human CRP (C-reactive protein) Antibody, 100μg

C-reactive protein (also known as CRP and Pentraxin 1 (PTX1)), is a member of pentraxin protein family. Similar to other pentraxins, CRP is a pattern recognition molecule that is able to recognize pathogens and promote their clearance. CRP tends to form a non-covalently linked pentamer with each subunit made up of two antiparallel β-sheets and a single short α-helix. It is characterized by the presence of a cleft that extends from the center of the subunit to its edge at the central pore of the pentamer structure. It has been demonstrated that CRP binds to the phosphocholine moieties expressed on the surface of dead or dying cells and some bacteria. This leads to the activation of the complement system and promotion of phagocytosis by macrophages to clear necrotic and apoptotic cells. CRP is secreted by hepatocytes and is a biomarker for inflammation. IL-6 has been shown to be an effective inducer of this protein. Besides binding to apoptotic cells, CRP also binds to a variety of molecules including C1q, bacterial polysaccharide, CD64, CD32, APCS, HDL, and fibronectin. In addition to participating in immune responses, CRP is also a prominent partaker in endothelial dysfunction and atherosclerosis. It was shown that CRP may exhibit direct proantherogenic effects by upregulating angiotensin type I receptor in smooth muscle cells and promoting their migration, proliferation, neointimal formation, and reactive oxygen species production. Mature human CRP shares 71% amino acid sequence homology with its mouse counterpart.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CRP/PTX1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 8 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For IHC-P, use 100 mM Tris, 10 mM EDTA, pH 9.0 for antigen retrieval buffer. Not recommended for use with Tris-buffered saline antigen retrieval buffer.
RRID: AB_2686984 (BioLegend Cat. No. 696006)
Structure: Monomer and homopentamer
Distribution: Hepatocytes
Function: Induces expression of E-selectin and VCAM-1 expression in epithelial cells. CRP binds to target, initiates, and regulates complement activation. Induced by IL-1 and IL-6.
Interaction: Phosphocholine on the surface of apoptotic cells
Ligand/Receptor: Interacts with C1q, bacterial polysaccharide, CD64, CD32, APCS (PTX2), HDL, leptin, and fibronectin.
Biology Area: Cell Biology
Antigen References: 1. Ansar W, Ghosh S. 2013. Immunol. Res. 56:131. 2. Agrawal A, et al. 2009. Adv. Exp. Med. Biol. 653:98. 3. Garlanda C, et al. 2005. Annu. Rev. Immunol. 23:337. 4. Verma S, et al. 2004. Circulation 109:1914. 5. Wadham C, et al. 2004. Circulation 109:2116. 6. Li XA, et al. 1998. Biochem. Biophys. Res. Commun. 244:249. 7. Lu J, et al. 2012. Immunol. Rev. 250:230. 8. Chen K, et al. 2006. Nat. Med. 12:425.
Gene ID: 1401
UniProt: View information about CRP on UniProt.org
Clone: A16065C
Regulatory Status: RUO
Other Names: C-reactive protein (CRP), Pentraxin 1 (PTX1)
Isotype: Mouse IgG1, κ