Biolegend, 696302, Purified anti-Mesothelin Antibody, 100μg

Mesothelin (MSLN) was initially cloned from a library of human pancreatic cancer cells. It was also identified by a monoclonal antibody, MAb K1, which recognized a glycoprotein present on the surface of mesothelial cells. The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The C-terminal glycosylphosphatidylinositol (GPI) anchor addition signal is removed (cleavage site: Ser598) and the latter replaced with a GPI anchor. The MSLN precursor is cleaved by furin at Arg295 into two products, a ~31 kD mature megakaryocyte potentiating factor (MPF residues Ser34 - Arg286) and a ~40 kD GPI-anchored membrane-bound mature mesothelin starting from Glu296. The 40 kD GPI-anchored mesothelin can be released by treatment with phosphatidylinositol-specific phospholipase C. Soluble mesothelin specifically binds to ovarian carcinoma cell line OVCAR-3, mediated by the cancer antigen 125 (CA125), a mucin-like glycoprotein on the surface of tumor cells. CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma, and it has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. Also, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates. Mesothelin is highly expressed in several human cancers including malignant mesothelioma, pancreatic, ovarian, cholangiocarcinoma, and lung adenocarcinoma.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human recombinant mesothelin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Optimal concentration of usage is 0.1 - 0.5 µg/mL for WB. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. The optimal concentration should be determined by titration for each individual assay of interest.
Application Notes: Precursor: 70 kDa. Mature form (N-glycosylated): 46-48kDa. Clone A15125D does not cross react with mouse (in-house tested).
RRID: AB_2687055 (BioLegend Cat. No. 696302)
Structure: The human MSLN gene encodes a ~71 kD precursor protein of 622 amino acids. The MSLN precursor is cleaved into two products, a ~31 kD mature megakaryocyte potentiating factor and a ~40 kD GPI-anchored membrane-bound mature mesothelin
Distribution: Mesothelial cells lining the pleura, peritoneum, and pericardium and tumor cells
Function: Cell adhesion; may promote cancer cell survival, proliferation, and metastasis; confers resistance to cytotoxic drug-induced apoptosis
Interaction: Cancer cells
Ligand/Receptor: CA125/MUC16
Biology Area: Cancer Biomarkers, Cell Adhesion, Cell Biology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Kojima T, et al. 1995. J. Biol. Chem. 270:21984. 2. Chang K and Pastan I. 1996. Proc. Natl. Acad. Sci. USA 93:136. 3. Rump A, et al. 2004. J. Biol. Chem. 279:9190. 4. Bharadwaj U, et al. 2011. Mol. Cancer 10:106. 5. Chen SH, et al. 2013. Sci. Rep. 3:1870. 6. Tang Z, et al. 2013. Anticancer Agents Med. Chem. 13:276.
Gene ID: 10232
UniProt: View information about Mesothelin on UniProt.org
Clone: A15125D
Regulatory Status: RUO
Other Names: Antigen recognized by antigen K1, megakariocyte potentiating factor (MPF), soluble MPF, mesothelin related protein
Isotype: Mouse IgG2b, κ