Biolegend, 697102, Purified anti-PIK3R1 Antibody, 100μg

Phosphatidylinositol 3-kinase (PI3K) is a heterodimer comprised of a catalytic subunit encoded by PIK3CA (p110α) and one of a number of regulatory subunits. PIK3R1, also known as PI3K p85-alpha, is an 85kD regulatory subunit of PI3K. Upon activation of the receptor tyrosine kinases, PI3K phosphorylates inositol lipids to phosphatidylinositol-3,4,5-trisphosphate (PIP3), subsequently leads to activation of AKT and downstream effectors. The PI3K signaling regulates various cellular processes such as protein synthesis, cell growth, cell cycle progression, cell proliferation, angiogenesis, and survival. In addition, PI3K pathway has been found to play important roles in tumorigenesis and cancer progression. PIK3R1 appears to be a tumor suppressor due to its ability to stabilize the catalytic subunit of PI3K. Mutations in PIK3R1 have been associated with the autosomal dominant disorder SHORT syndrome.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human PIK3R1 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 5.0 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody may not react with the p85 beta based on sequence homology.
RRID: AB_2687110 (BioLegend Cat. No. 697102)
Structure: 724 amino acids with a predicted molecular weight of 83.6 kD. Contains an N-terminal SH3 domain, a Rho-GAP domain, and two C-terminal SH2 domains.
Distribution: Nucleus, cytoplasm, plasma membrane, ER membrane.
Function: PIK3R1 is a regulatory subunit of phosphatidylinositol 3-kinase and is involved in the signaling regulating protein synthesis, cell growth, cell cycle progression, cell proliferation, angiogenesis, and survival.
Interaction: Forms a heterodimer with the catalytic subunit of PIK3. Interacts with CSF1R, PIK3R2, XBP1, FER, TEK, PTK2, TOM1L1, LIME1, SOCS7, RUFY3, LYN, CBLB, IRS1, IRS4, FGR, HCK, ERBB4, and NTRK1.
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Munkley J, et al. 2015. Oncoscience. 2:755. 2. Huang XP, et al. 2015. FEBS J. 282:579. 3. Wei Y, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:6829. 4. Najib S, et al. 2012. Mol. Cell. Biol. 32:1004. 5. Anderson DH. 2010. Cell Cycle. 9:2055. 6. De Gregorio G, et al. 2007. Oncogene. 26:2039.
Gene ID: 5295
UniProt: View information about PIK3R1 on UniProt.org
Clone: W16101A
Regulatory Status: RUO
Other Names: Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, GRB1,
Isotype: Rat IgG2a, κ