Biolegend, 699001, Purified anti-NTAL (LAT2) Antibody, 25μg

Non-T cell activation linker (NTAL) functions as transmembrane adaptor scaffold that links engagement of antigen receptor binding to downstream effector molecules, playing an essential role in maturation, activation, and differentiation in various cell types. It is important for BCR (B-cell antigen receptor) and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-signaling in B-cells and myeloid cells, respectively, and plays a critical role in FCER1 (high affinity immunoglobulin epsilon receptor) signaling in mast cells.  NTAL is encoded by the LAT2 gene, which is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NTAL recombinant protein (27-243 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-5000). For flow cytometric staining, the suggested use of this reagent is ≤0.00375 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not cross-react with mouse NTAL (in-house tested). Daudi cells were selected for antibody testing due to the cell line’s low expression level of the mRNA encoding NTAL (source: Human Protein Atlas).
RRID: AB_2715811 (BioLegend Cat. No. 699001) AB_2715812 (BioLegend Cat. No. 699002)
Structure: 243 amino acids with a predicted molecular weight of 26.5 kD, palmitoylation moiety permits localization to lipid rafts or tetraspanin-enriched microdomains.
Distribution: Plasma membrane
Function: NTAL is extensively phosphorylated on multiple tyrosine residues following activation, enabling the protein to serve as a scaffold for multiple signaling complexes in various cell contexts. May also be post-translationally regulated by Caspase-3-mediated proteolytic cleavage in human B cells and monocytes.
Interaction: Phospholipase C gamma 1 and 2. When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1, and CBL.
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Arbulo-Echevarria MM, et al. 2016. J. Leukoc Biol. 100:351. 2. Stepanek O, et al. 2014. Cell Signal. 5:895. 3. Orr Sj, et al. 2013. Plos Pathog. 9: e1003357. 4. Halova I, et al. 2013. J. Biol. Chem. 288:9801. 5. Duque-Alfonso J, et al. 2011. Oncogene. 30:3062
Gene ID: 7462
UniProt: View information about NTAL on UniProt.org
Clone: W15102A
Regulatory Status: RUO
Other Names: NTAL, LAT2, WBS15, LAB
Isotype: Mouse IgG1, κ