Biolegend, 699306, Alexa Fluor® 488 anti-Vimentin Antibody, 100μg

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes 1,2 . Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells 3 . Increased vimentin expression is frequently used as an EMT marker in cancer 4 . Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus 5 and rheumatoid arthritis 6 , and also found after transplantation 7 .
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial mouse Vimentin (224-446 a.a.) recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This antibody does not cross-react with human (in-house tested). May be weakly reactive with human Vimentin by WB.
RRID: AB_2888889 (BioLegend Cat. No. 699305) AB_2888889 (BioLegend Cat. No. 699306)
Structure: 466 amino acids with a predicted molecular weight of 54 kD.
Distribution: Cytoplasm.
Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Interaction: HCV core protein, LGSN, SYNM, PLEC, SLC6A4, STK33, LARP6, RAB8B, TOR1A, TOR1AIP1, and BCAS3.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Kidd ME, et al. 2014. Am J Respir Cell Mol Biol. 50:1. 2. Fuchs E, et al. 1994. Annu Rev Biochem. 63:345. 3. Zeisberg M, et al. 2009. J Clin Invest. 119:1429. 4. Scanlon CS, et al. 2013. J Dent Res. 92:114. 5. Thebault S, et al. 2002. J Immunol. 169:4046. 6. Vossenaar ER, et al. 2004. Arthritis Res Ther. 6:R142. 7. Rose ML. 2013. Hum Immunol. 74:1459.
Gene ID: 22352
UniProt: View information about Vimentin on UniProt.org
Clone: W16220A
Regulatory Status: RUO
Other Names: Vim, CTRCT30, HEL 113, Epididymis Luminal Protein 113
Isotype: Rat IgG2a, κ