Biolegend, 699902, Epitope Tag Big Motif Antibody Sampler Kit, 1kit

The Epitope Tag Big Motif Antibody Sampler Kit provides a convenient resource to detect big Epitope Tag fused protein by Western Blot. GST is commonly used as a tag to create fusion proteins in order to facilitate purification and detection of these proteins of interest. Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems. mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. Thioredoxin is an 11.7 kD E. coli cytoplasmic protein. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation. For additional antigen details, please refer to individual product datasheets.
1kit
Formulation: Please refer to individual product datasheets for details.
Preparation: All antibodies in this kit were purified by affinity chromatography.
Concentration: All antibodies in the kit are at 0.5mg/ml
Storage & Handling: Upon receipt, store undiluted at 2-8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows: Anti-GST: 0.05-0.5 µg/ml Anti-GFP: 0.05-0.5 µg/ml Anti-mCherry: 0.1-1 µg/ml Anti-Thioredoxin 1: 0.05-0.5 µg/ml It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For verified or reported applications for these antibodies, please see individual product datasheets.
Application References(PubMed link indicates BioLegend citation): Nicol C, et al. 2011. Biochem. Biophys. Res. Commun. 405:417. PubMed (Anti-GST)
Biology Area: Cell Biology
Gene ID: NA
Regulatory Status: RUO
Other Names: Glutathione-S-transferase, Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA, Thioredoxin Tag, DSRED, Green fluorescent protein, GFP