Biolegend, 768914, Biotinylated Recombinant Human FAP (carrier-free), 25μg

Fibroblast activation protein (FAP) is a cell-surface transmembrane-anchored dimeric proteas. FAP expression is very low in adult organs but is upregulated by activated fibroblasts at the sites of tissue remodeling, including fibrosis, atherosclerosis, arthritis and tumors. FAP is broadly expressed on cancer associated fibroblasts (CAFs), which are a major component of the stromal microenvironment of carcinomas. FAP has been identified as a promising immunotherapy target antigen for epithelial carcinomas such as prostate, lung, pancreatic cancer and mesothelioma. It has been shown that by removing the fibrosis barrier, ‘FAP-Chimeric Antigen Receptor-Macrophages’ enhanced the penetration of gemcitabine and immune cells, improved PDAC (pancreatic ductal adenocarcinoma) sensitivity to chemotherapy and immunotherapy and significantly prolonged survival. FAP is not commonly expressed by blood vessels within healthy tissues or by normal endothelial cells (ECs) but can be strongly and consistently induced on ECs within the glioblastoma microenvironment. FAP directly cleaves CXCL12 to inactivate canonical wnt signaling and therefore plays a direct role in regulating osteogenic and adipogenic differentiation of mesenchymal progenitor cells.
25μg
Source: Human FAP, amino acids Leu26-Asp760 (Accession # Q12884), with a C-terminal 6His tag and Avi tag was expressed in CHO cells. Human FAP was site-specifically biotinylated by BirA.
Molecular Mass: The 761 amino acid recombinant protein has a predicted molecular mass of approximately 87.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 80-90 kD in SDS-PAGE.
N-terminalSequence Analysis: The predicted N-terminal amino acid is Leu.
Purity: > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in 16 mM Tris, 240 mM NaCl, 20% Glycerol, pH 7.5
Endotoxin Level: Less than 0.1 EU per μg of protein as determined by the LAL method
Concentration: 25 µg and 100 µg at 1.0 mg/mL
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min.
Application: Bioassay
Application Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].
Structure: Monomer
Distribution: Adipose tissue and Cancer associated fibroblasts (CAFs)
Function: Tissue remodeling, fibrosis, wound healing, inflammation, tumor growth and metastasis
Interaction: Serpin F2 (alpha-2-antiplasmin); Gelatin (Type I collagen)
Bioactivity: Biotinylated human FAP cleaves a fluorogenic substrate Z-Gly-Pro-AMC with a specific activity value > 1800 pmol/µg/min.
Biology Area: Cancer Biomarkers
Molecular Family: Enzymes and Regulators
Antigen References: Ostermann E. et al. 2008. Clin Cancer Res.14:4584. Kakarla S. et al. 2013. Mol Ther. 21:1611. Gulati P. et al. 2018. Clin Cancer Res. 24:3981. Freedman JD. et al. 2018. Cancer Res. 78: 6852. Schuberth PC. et al. 2013. J Transl. Med. 11:187 Garin‐Chesa P. et al. 1990. Proc Natl Acad Sci USA. 87:7235. Nurmik M. et al. 2020. Int J Cancer. 146:895. Wang W. et al. 2025. J Control Release. 384:113888
Gene ID: 2191
UniProt: View information about FAP on UniProt.org
Regulatory Status: RUO
Other Names: FAP, FAP-alpha, Fibroblast activation protein alpha, Integral membrane serine protease, Seprase, DPP4