Biolegend, 801103, Purified anti-GFAP Antibody, 25μg

Glial Fibrillary Acidic Protein (GFAP) is a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. Astrocytes respond to many damage and disease states resulting in "astrogliosis" or the presence of a "glial response". GFAP antibodies are widely used to see the reactive astrocytes which form part of this response, since reactive astrocytes stain much more strongly with GFAP antibodies than normal astrocytes. GFAP also forms a major component of the so-called glial scar, an astrocyte rich structure apparently forming part of the barrier to nerve fiber regeneration following damage in the central nervous system. Neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of normal and reactive astrocytic cells and neural stem cells. This antibody was raised against a preparation of purified pig spinal core GFAP.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: PBS, 50% glycerol, 5mM NaN3 as supplied by vendor.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C for up to one year.
Application: IHC-P - Quality testedWB - VerifiedICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/mL for chromogenic staining and 2.0 - 10 µg/mL for fluorescent staining is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.2 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Filipowicz AR, et al. 2016. Sci Rep. 6: 32900. PubMed (IHC-P) Edamakanti CR, et al. 2018. J. Clin Invest. 128(6):2252 (ICC) de Kloet AD, et al. 2016. Brain Struct Funct. 221(2):891 (IHC-F) Hawkins KE, et al. 2014. J Neurochem. 129(1):130 (WB) Zhao WN, et al. 2012. J Biomol Screen. 17(9):1252 (ICC)
Product Citations: Tao W, et al. 2020. EMBO Mol Med. 12:e12291. PubMed
RRID: AB_2810699 (BioLegend Cat. No. 801103)
Structure: GFAP is a 432 amino acid protein with a molecular mass of ~50 kD
Distribution: Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin. Cellular distribution: Cytoskeleton and cytosol
Function: GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS
Antigen References: van Bodegraven EJ, et al. 2019. Glia. 10.1002/glia.23594. Pekny M, et al. 2019. Neurosci Lett. 689:45. Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12).
Gene ID: 26701458024387
UniProt: View information about GFAP on UniProt.org
Clone: MCA-5C10
Regulatory Status: RUO
Other Names: GFAP, Glial fibrillary acidic protein
Isotype: Mouse IgG1, κ