Biolegend, 803102, Anti-Alpha-I Spectrin Antibody (Previously Covance catalog# SIG-39700), 500microl

Spectrin is an intracellular cytoskeletal protein that provides structure to eukaryotic cells and helps maintain plasma membrane integrity.  Alpha-1 Spectrin, encoded by the SPTA1 gene, is found primarily in erythrocytes and in the cerebellum.
500microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant α spectrin SH3 domain
Formulation: Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in Western Blotting (WB). Additional reported applications include immunocytochemistry1, 2. Clone 17C7 is a specific marker for human, rat and mouse alpha I spectrin. It reacts with erythroid spectrin in red blood cells and in cerebellum. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 280 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes1, 2. This antibody was developed against a recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
Application References(PubMed link indicates BioLegend citation): Xu J, et al. 2001. Brain Res. 898(1):171-7. (WB, ICC) Xu J, et al. 2000. J. Cell Sci.113:3805-14. (WB, ICC)
Product Citations: Lubbers ER, et al. 2019. J Biol Chem. 294:9576. PubMed
RRID: AB_2564659 (BioLegend Cat. No. 803101)
Structure: Expected MW: 250 kD
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Presynaptic proteins, Scaffold Proteins
Antigen References: De Matteis MA, Morrow JS. 2000. J. Cell Sci. 113:2331-43. Bennett V, Gilligan DM. 1993. Annu. Rev. Cell Biol. 9:27-66.
Gene ID: 670820739289257
UniProt: View information about alpha-I Spectrin on UniProt.org
Clone: 17C7
Regulatory Status: RUO
Other Names: EL2, SPTA1, Spectrin alpha chain, erythrocyte, alpha-I spectrin, erythroid alpha-spectrin
Previously: Covance Catalog# SIG-39700Signet Catalog# 9700-02Signet Catalog# 9700-05Signet Catalog# 9700-10
Isotype: Mouse IgG1, κ