Biolegend, 803201, Anti-Alpha-II Spectrin Antibody (Previously Covance catalog# SIG-39702), 200microl

The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
200microl
Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Preparation: Ascites
Storage & Handling: Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:2000 - 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution of 1:1000 is suggested. For immunocytochemistry, a dilution of 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain. This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation. Reactivity to Drosophila was only verified with the purified format.
Application References(PubMed link indicates BioLegend citation): Konieczny P, et al. 2008. J Cell Biol. 181:667-81 Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF) Xu J, et al. 2000. J Cell Science. 113:3805-14 De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43 Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427 Goodman SR, et al. 1995. Brain Res Bull. 36:593-606 Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580 Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66 Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed
Product Citations: Wang G et al. 2019. eLife. 8 pii: e38730. PubMed Miazek A, et al. 2021. Scientific Reports. 11(1):7312. PubMed Torii T, et al. 2020. The Journal of Cell Biology. 219(2):e201907048. PubMed Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11323-11334 . PubMed Zhou R, et al. 2022. Nat Commun. 13:3196. PubMed Lubbers ER, et al. 2019. J Biol Chem. 294:9576. PubMed Hamdan H, et al. 2020. Nat Commun. 0.527777778. PubMed Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11311-11322 . PubMed Liu Y, et al. 2019. Sci Adv. 5:eaav7803. PubMed Cousin MA, et al. 2021. Nat Genet. 53:1006. PubMed
RRID: AB_2564660 (BioLegend Cat. No. 803201)
Structure: Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Presynaptic proteins, Scaffold Proteins
Antigen References: Pike BR, et al. 2001. J Neurochem. 78(6):1297-306. Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
Gene ID: 6709
UniProt: View information about alpha-II Spectrin on UniProt.org
Clone: D8B7
Regulatory Status: RUO
Other Names: Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
Previously: Covance Catalog# SIG-39702Signet Catalog# 9702-02Signet Catalog# 9702-05Signet Catalog# 9702-10
Isotype: Mouse IgG2b, κ