Biolegend, 808603, Biotin anti-β-Amyloid, 1-38 Antibody (Previously Covance catalog# MRB-5215), 50microl

Soluble Aβ (Amyloid-beta, beta-amyloid, or Abeta) peptides, 36 to 43 amino acids in length, are natural proteolytic cleavage products of amyloid precursor protein (APP). However, soluble abnormally aggregated Aβ species are associated with Alzheimer's disease (AD), can be toxic to neurons, and insoluble Aβ aggregates are the main component of cerebral amyloid plaques, which are a pathological hallmark of AD.
50microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rabbit
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application: ELISA, WB, IHC ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. The optimal working dilution should be determined for each specific assay condition. • WB: 1:1,000 - 1:2,000* • IHC: 1:50 - 1:100 Tissue: Formalin-fixed Paraffin-embedded Tissues Pre-treatment: 70% Formic Acid for 20 minutes at room temperature Primary incubation: 24 hours at 2-4°C • ELISA: Can be used as capture antibody in a sandwich ELISA with 4G8 detection (Ref Cat# 800707 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, HRP Labeled, or Cat No. 800701 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody). For capture antibody, recommended usage is between 1.0 and 10.0 µg/ml. Recommended blocking buffer is 0.5-1.0% BSA in PBS. If using Cat No. 800707 for detection; recommended usage is between 0.25-1.0 µg/ml.
Application Notes: This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA. *Expected MW: 4 kD WB Positive Control: Alzheimer's Brain Lysate IHC Positive Control: Alzheimer's Brain Tissue This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform that ends at the 38th amino acid.
Application References(PubMed link indicates BioLegend citation): Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA 28:10323. ((F) Giaccone G, et al. 2012. Acta. Neuropathol. 124:809. (IHC, IF, WB)
Product Citations: Ma C, et al. 2021. Front Immunol. 11:582998. PubMed
RRID: AB_2564746 (BioLegend Cat. No. 808603)
Disclaimer: Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: APP/β-Amyloid
Gene ID: 351
UniProt: View information about beta-Amyloid 1-38 on UniProt.org
Clone: 7-14-4
Regulatory Status: RUO
Other Names: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously: Covance Catalog# MRB-5215
Isotype: Rabbit Ig
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.