Biolegend, 811501, Purified anti-Clathrin Light Chain (CLTA) Antibody (Previously Covance catalog# MMS-423P), 100microl

Clathrin consists of three heavy chains (192kD). The three light chains (LCa or LCb, 25-29kD) self-assembles into a polyhedral cytoskeletal element. The self-assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against a peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains, residues 23-44.
Formulation: Phosphate-buffered solution + 0.03% Thimerosal.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: Store frozen (-20 or -80°C); shipped on blue ice. Aliquot upon receipt before freezing. Avoid repeated freeze/thaw cycles.
Application: IHC-P - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Zlatic SA, et al. 2013. Mol Biol Cell. 24:2378-88. Jiang R, et al. 2000. J Biol Chem. 275(12):8439-47. Nathke IS, et al. 1992. Cell. 68:899-910. Jackson AP, et al. 1987. Nature. 326:154-9.
RRID: AB_2564758 (BioLegend Cat. No. 811501)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID: 1211
UniProt: View information about Clathrin Light Chain on UniProt.org
Clone: CON.1
Regulatory Status: RUO
Other Names: Clathrin light chain A, clathrin, light polypeptide (Lca)
Previously: Covance Catalog# MMS-423P
Isotype: Mouse IgG2b, κ