Biolegend, 814701, Purified anti-RhoG Antibody (Previously Covance catalog# MMS-5032), 100microl

RhoG (Ras homology Growth-related) is also known as ARGH, belonging to the Rho family of small G proteins capable of signaling via binding GTP and catalyzing it to GDP. It is expressed widely in mammalian cells, including fibroblasts, leukocytes, neuronal cells, endothelial cells, and on cell lines, such as HeLa cells. RhoG is involved in a number of signaling pathways, including those for chemokine receptors in cell motility, gene transcription, endocytosis, neurite outgrowth, and others.
100microl
Verified Reactivity: Human, Rat, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide corresponding to amino acids 161-180 of the RhoG protein conjugated to KLH.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 4.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Meller J, et al. 2008. J Cell Science 121:1981-1989. (WB)
RRID: AB_2564786 (BioLegend Cat. No. 814701)
Structure: Expected MW: 18 kD
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Postsynaptic proteins
Gene ID: 391
UniProt: View information about RhoG on UniProt.org
Clone: 1F3 B3 E5
Regulatory Status: RUO
Other Names: ARHG, rho-related GTP-binding protein RhoG, ras homolog gene family, member G (rho G), Rho-G, Rho G
Previously: Covance Catalog# MMS-5032
Isotype: Mouse IgG2b, κ