Biolegend, 814802, Purified anti-p62 (SQSTM1) Antibody (Previously Covance catalog# MMS-5034), 25microl

The p62 protein, also called sequestosome 1 (SQSTM1), is a ubiquitin-binding scaffold protein that colocalizes with ubiquitinated protein aggregates in many neurodegenerative diseases and proteinopathies of the liver. The protein is able to polymerize via an N-terminal PB1 domain and can interact with ubiquitinated proteins via the C-terminal UBA domain. Also, p62/SQSTM1 binds directly to LC3 and GABARAP family proteins via a specific sequence motif. The protein is itself degraded by autophagy and may serve to link ubiquitinated proteins to the autophagic machinery to enable their degradation in the lysosome. Recent studies have revealed a novel function for p62 in innate immunity.
25microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide sequence corresponding to amino acids 201-212 of the human SQSTM 1 (isoform 1) protein conjugated to KLH.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 10 - 15 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), immunoblotting (WB), and immunocytochemistry (ICC).
Product Citations: Zhou B, et al. 2020. Nat Microbiol. 5:1576. PubMed Tapia D, et al. 2019. Nat Commun. 10:735. PubMed Trejo-Lopez JA, et al. 2020. J Neuropathol Exp Neurol. 407:79. PubMed Quach C, et al. 2019. Nat Commun. 4.361805556. PubMed
RRID: AB_2728534 (BioLegend Cat. No. 814802) AB_2564787 (BioLegend Cat. No. 814801)
Structure: p62 (SQSTM1) is a 440 amino acid protein with an expected molecular mass of 62 kD.
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family: Adaptor Proteins, Autophagosome Markers
Gene ID: 8878
UniProt: View information about p62 on UniProt.org
Clone: 1B5.H9
Regulatory Status: RUO
Other Names: P60, p62, A170, OSIL, PDB3, ZIP3, p62B, sequestosome-1, oxidative stress-induced-like ubiquitin-binding protein p62
Previously: Covance Catalog# MMS-5034
Isotype: Mouse IgG1, κ