Biolegend, 818002, Purified anti-Tyrosine Hydroxylase Antibody (Previously Covance catalog# MMS-5210, MMS-5210-100, MMS5210), 25microl

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.
25microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality testedWB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).This antibody is less reactive in human tissue in Western Blot.
Product Citations: Harraz MM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200545119. PubMed Harraz MM, et al. 2021. Mol Psychiatry. 26:370. PubMed Shi L, et al. 2021. Translational Neurodegeneration. 10(1):13. PubMed Marfull-Orom P, et al. 2021. Journal of Neurochemistry. . PubMed
RRID: AB_2734573 (BioLegend Cat. No. 818002) AB_2564816 (BioLegend Cat. No. 818001)
Structure: Expected MW: 65 kD
Cell Type: Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family: Enzymes and Regulators, Presynaptic proteins
Gene ID: 25085
UniProt: View information about Tyrosine Hydroxylase on UniProt.org
Clone: 2/40/15
Regulatory Status: RUO
Other Names: Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Previously: Covance Catalog# MMS-5210, MMS-5210-100, MMS5210
Isotype: Mouse IgG2a, κ