Biolegend, 819411, Purified anti-Histone H3 (C-terminus) Antibody, 25μg

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
25μg
Verified Reactivity: Human, Mouse, Rat, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation: 50 mM sodium citrate, 150mM sodium chloride, pH 5.8 with stabilizer
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration of 1.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.01 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Reactivity to Drosophila was only verified with the purified format.
Additional Product Notes: For Western blotting, the suggested use of this reagent is 0.01 - 0.2 µg/mL in human, mouse, and rat and 0.1 - 0.5 µg/mL in Drosophila.
Product Citations: Liu PS, et al. 2023. Nat Immunol. 24:452. PubMed Reers AB, et al. 2023. Epigenetics Chromatin. 16:25. PubMed Lu H, et al. 2022. Nucleic Acids Res. 50:5635. PubMed Bado IL, et al. 2021. Developmental Cell. 56(8):1100-1117.e9. PubMed
RRID: AB_2820127 (BioLegend Cat. No. 819411) AB_2820128 (BioLegend Cat. No. 819412)
Structure: Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution: Nucleus
Function: Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction: Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 1B1-B2
Regulatory Status: RUO
Other Names: Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype: Mouse IgG3, κ