Biolegend, 824004, Biotin anti-CX3CR1 Antibody, 100μg

CX3C chemokine receptor 1 (CX3CR1) also known as fractalkine receptor or G-protein coupled receptor 13, is a receptor that binds the chemokine CX3CL1. CX3CR1 expression is associated with T cells, NK cells, and brain microglia. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediates cell adhesion and migration. CX3CL1 has been reported to play an important role in neuroinflammation and neurodegeneration.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 207-222 of the human CX3CR1 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 20 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody is effective in Immunoblotting (WB) and immunohistochemistry (IHC) for the relevant formats of this clone.
RRID: AB_2650684 (BioLegend Cat. No. 824004)
Structure: CX3CR1 is a 355 amino acid G-protein coupled receptor, 7 transmembrane regions protein with a molecular mass of 40 kD.
Distribution: Tissue Distribution: central nervous system, and blood. Cellular Distribution: plasma membrane.
Function: CX3CR1 is involved in cell recruitment during inflammation, and participates in cell adhesion and extravasation from blood vessels. It is also known as a coreceptor for HIV1.
Ligand/Receptor: CX3CL1 (fractalkine, neurotactin).
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Chen P, et al. 2016. BioMed. Res. Int. 2016. 8090918. PubMed 2. Sheridan GK, Murphy KJ. 2013. Open Biol. 3 (12) 130181. PubMed
Gene ID: 1524
UniProt: View information about CX3CR1 on UniProt.org
Clone: 8E10.D9
Regulatory Status: RUO
Other Names: CX3C chemokine receptor 1, fractalkine receptor, beta chemokine receptor-like 1, chemokine (C-C) receptor-like 1, GRP13, V28
Isotype: Mouse IgG1, λ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.