Biolegend, 827701, Purified anti-Histone H4 Trimethyl (Lys 20) Antibody (Previously Covance catalog# MMS-5288), 100microl

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide conjugated to KLH containing trimethylated lysine 20 of human Histone H4
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/mL
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Botuyan MV, et al. 2006. Cell. 127:1361. (WB)
Product Citations: Wimmers F, et al. 2021. Cell. 184:3915. PubMed
RRID: AB_2564914 (BioLegend Cat. No. 827701)
Structure: Histone proteins H3 and H4 bind to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution: Histone H4 is ubiquitously expressed in all cells.
Function: Transcription regulation, DNA repair, DNA replication, chromosomal stability, silencing of repetitive DNA and transposons.
Interaction: Various transcription factors.
Biology Area: Cancer Biomarkers, Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: 1. Chen HM, et al. 2015. Epigenetics and Dermatology 409. 2. Bhasin M, et al. 2006. J. Comput. Biol. 13:102. 3. Nelson DM, et al. 2016. Genome Biol. 17:158.
Gene ID: 8359
UniProt: View information about Histone H4 on UniProt.org
Clone: 6F8-D9
Regulatory Status: RUO
Other Names: Histone H4, histone 1, H4a, H4 histone family, member A, H4K20me3
Previously: Covance Catalog# MMS-5288
Isotype: Mouse IgG1, κ