Biolegend, 828801, Purified anti-PP2AC Methyl (Leu 309) Antibody (Previously Covance catalog# MMS-5117), 100microl

PP2AC (Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform), encoded by the PPP2CA gene, is the main phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2AC is reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). This carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, which in turn, modulates the PP2A holoenzyme's enzyme activity and cellular localization.
100microl
Verified Reactivity: Yeast
Reported Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a synthetic peptide from Human methylated Leucine 309 PP2A catalytic subunit.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. The optimal working dilution should be determined for each specific assay condition. • WB: 1:100 - 1:800*
Application Notes: This antibody is effective in immunoblotting (WB). * Expected MW: 36 kD WB Positive Control: BY4741 yeast cells The immunogen sequence (C-ßA-RRTPDYFL-Ome) is conserved from human, mouse to yeast.
Product Citations: Padi SKR, et al. 2024. Nature. 195:625. PubMed Vit G, et al. 2022. EMBO J. 41:e110611. PubMed
RRID: AB_2564923 (BioLegend Cat. No. 828801)
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID: 5515
UniProt: View information about PP2AC on UniProt.org
Clone: 2A10
Regulatory Status: RUO
Other Names: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PPP2CA
Previously: Covance Catalog# MMS-5117
Isotype: Mouse IgG1, κ