Biolegend, 830501, Purified anti-mouse CD18 Antibody (Previously Covance catalog# MRT-5214), 100microl

CD18 is a 95 kD protein, also known as integrin β 2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and α X β 2 , respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. The M18/2 antibody is able to block tumor cell metastasis and enhance cell adhesion.
100microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: This monoclonal antibody was raised against cell membrane glycoproteins derived from T-cell lymphoma BW5147 cells.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: FC
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The optimal working dilution should be determined for each specific assay condition. • FC: 1:1000 - 1:10,000*
Application Notes: This antibody is effective in flow cytometry (FC). *FC Positive Control: Mouse blood Rat IgG2a Isotype antibody available: Catalog number: 910601
Application References(PubMed link indicates BioLegend citation): Trowbridge IS, et al. Molecular Complexity of Leukocyte Surface Glycoproteins Related to the Macrophage Differentiation Antigen MAC-1. J. Exp. Med. 154: 1517-1524, November 1981.
RRID: AB_2564939 (BioLegend Cat. No. 830501)
Biology Area: Cell Biology, Complement, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Wilson RW, et al. 1993. J. Immunol. 151:1571.
Gene ID: 16414
UniProt: View information about CD18 on UniProt.org
Clone: C71/16
Regulatory Status: RUO
Other Names: Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527
Previously: Covance Catalog# MRT-5214
Isotype: Rat IgG2a, κ