Biolegend, 836506, Alexa Fluor® 488 anti-Myelin Basic Protein Antibody, 100μg

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence. SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.
Application References(PubMed link indicates BioLegend citation): Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC) Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P) Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB) McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F) Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P) Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC) Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P) Rusielewicz T, et. al. 2014. Glia 62:580. (ICC) Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC) Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P) Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC) Zhao X, et al. 2010. Neuron. 65:612. (ICC)
RRID: AB_2801174 (BioLegend Cat. No. 836505) AB_2801175 (BioLegend Cat. No. 836506)
Structure: Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution: Cytoskeleton, plasma membrane, cytosol, and extracellular.
Function: Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID: 4155
UniProt: View information about Myelin Basic Protein on UniProt.org
Clone: SMI 94
Regulatory Status: RUO
Other Names: Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype: Mouse IgG1, κ