Biolegend, 840001, Anti-GFAP Antibody (Previously Covance catalog# PRB-571C), 100microl

Glial Fibrillary Acidic Protein (GFAP) was found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. The GFAP protein runs on gels at ~55kD protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
100microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: This antiserum was made with a preparation of recombinant GFAP expressed in bacteria and highly purified. Subsequent boosts were performed with purified GFAP from bovine spinal cord.
Preparation: Serum
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application: WB - Quality testedIHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, a dilution range of 1:10000 - 1:20000 is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:1000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.
Additional Product Notes: This product may contain other non-IgG subtypes.
Product Citations: García Fallit M, et al. 2023. Viruses. 15:. PubMed Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed Zuliani I, et al. 2020. Neurotherapeutics. . PubMed Miyamoto Y, et al. 2016. Nat Commun. 7:13478. PubMed Fischer M, et al. 2021. Alcohol Clin Exp Res. 45:979. PubMed Zelenova EA, et al. 2021. Cells. 10:. PubMed
RRID: AB_2565444 (BioLegend Cat. No. 840001)
Structure: GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Distribution: Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin. Cellular distribution: Cytoskeleton and cytosol
Function: GFAP is a class III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Cell Type: Astrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: van Bodegraven EJ, et al. 2019. Glia. 67:1417-1433. Pekny M, et al. 2019. Neurosci Lett. 689:45. Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12).
Gene ID: 2670
UniProt: View information about GFAP on UniProt.org
Clone: Poly28400
Regulatory Status: RUO
Other Names: Glial Fibrillary Acidic Protein
Previously: Covance Catalog# PRB-571C
Isotype: Rabbit Polyclonal IgG