Biolegend, 844401, Purified anti-LRRK2 Antibody (Previously Covance catalog# MMS-5301), 100microl

Leucine-rich repeat kinase 2 (LRRK2), is a member of the leucine-rich repeat kinase family. This protein also functions as a GTPase. Proteins with leucine-rich regions play a role in protein-protein interactions and mediate assembly of the cell's structural framework (cytoskeleton). Mutations in LRRK2 genes have been associated with late-onset Parkinson's disease.
100microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Antibody is against amino acids 100 - 500 of human LRRK2.
Formulation: Phosphate-buffered solution.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1 mg/ml
Storage & Handling: This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Wang X, et al. 2021. Sci Rep. 11:12900. PubMed
RRID: AB_2750047 (BioLegend Cat. No. 844402) AB_2565614 (BioLegend Cat. No. 844401)
Structure: This gene is a member of the leucine-rich repeat kinase family and is encoded by the PARK8 gene. The protein is present mainly in the cytoplasm but can be associated with the mitochondrial outer membrane.Expected MW: >200 kD
Distribution: Tissue distribution: Lung, Kidney, parathyroid gland, CNS Cellular distribution: cytoplasm, mitochondrion, nucleus, endoplasmic reticulum, lysosome, Golgi apparatus, endosome
Function: LRRK2 interacts with the C-terminal R2 RING finger domain of parkin. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. Mutations in this gene have been associated with Parkinson disease.
Biology Area: Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Mitochondrial Markers
Antigen References: Paisán-Ruíz C, et al. 2004. Neuron. 44(4):595-600. Alegre-Abarrategui J, et al. 2009. Hum. Mol. Genet. 18(21):4022-34. Gómez-Suaga P, et al. 2012. Hum. Mol. Genet. 21(3):511–25. Orenstein SJ, et al. 2013. Nat. Neurosci. 16(4):394–406. Cherra SJ, et al. 2013. Am. J. Pathol. 182(2):474–84.
Gene ID: 120892
UniProt: View information about LRRK2 on UniProt.org
Clone: 8G10
Regulatory Status: RUO
Other Names: Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 17, AURA17, DARDARIN, PARK8, RIPK7, ROCO2
Previously: Covance Catalog# MMS-5301
Isotype: Mouse IgG1, κ