Biolegend, 847201, Purified anti-SOX-10 Antibody, 25μg

SOX-10 is a transcriptional activator that is involved in the regulation of embryonic development and in the determination of cell fate.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to the N-terminus of human SOX-10.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested SB - Community verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody may show weaker reactivity to human tissues.
Additional Product Notes: This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.
Product Citations: Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
RRID: AB_2734617 (BioLegend Cat. No. 847201) AB_2629666 (BioLegend Cat. No. 847202)
Structure: SOX-10 is a 466 amino acid protein with a molecular mass of 50 kD.
Distribution: Tissue distribution: primarily expressed in the nuclei of glia in fetal and adult brain, and in the heart, salivary gland and skin. Cellular distribution: nucleus.
Function: SOX-10 likely functions synergistically with the POU domain protein TST-1/OCT6/SCIP to confer function of other transcription factors in developing and mature glia. The protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development.
Interaction: DNA, PAX3, NMI.
Cell Type: Microglia
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Endosomal Markers
Antigen References: 1. Bondurand N, Sham MH. 2013. Dev. Biol. 382(1):330. PubMed 2. Mollaaghababa R, Pavan WJ. 2003. Oncogene. 22(20):3024. PubMed
Gene ID: 6663
UniProt: View information about SOX-10 on UniProt.org
Clone: BSB-62
Regulatory Status: RUO
Other Names: Transcription factor SOX-10
Isotype: Mouse IgG2b, κ
Q: If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
A: It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
Q: Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
A: Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
Q: Are other fluorophores compatible with IBEX?
A: Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
Q: The same antibody works in one tissue type but not another. What is happening?
A: Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
Q: How can I be sure the staining I’m seeing in my tissue is real?
A: In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.