Biolegend, 848001, Purified anti-mouse P2RY12 Antibody, 25μg

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse P2RY12.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For flow cytometric staining. a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research. Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.
Additional Product Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded (FFPE) tissues, we recommend antigen retrieval with either Sodium Citrate H.I.E.R. (Cat. No. 928502) or Retrieve-All Antigen Unmasking #3 (Cat. No. 927601). View more applications data for this product in our Scientific Poster Library.
Product Citations: Salvany S, et al. 2022. Brain Pathol. 32:e13078. PubMed Albertini G, et al. 2023. Mol Psychiatry. . PubMed He D, et al. 2022. Immunity. 55:159. PubMed Zhao Q, et al. 2022. Proc Natl Acad Sci U S A. 119:e2113310119. PubMed Chen S, et al. 2022. Acta Neuropathol Commun. 10:188. PubMed Gras S, et al. 2021. Aging (Albany NY). 13:18051. PubMed Manich G, et al. 2020. Front Cell Neurosci. 14:567404. PubMed Malsy J, et al. 2020. Elife. 9:00. PubMed Chen HR, et al. 2022. Theranostics. 12:512. PubMed Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed Shi X, et al. 2021. Nat Commun. 12:6943. PubMed Safaiyan S, et al. 2021. Neuron. 109(7):1100-1117.e10. PubMed Zhou X, et al. 2020. Nat Neurosci. 1.192361111. PubMed Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed Li Y, et al. 2021. J Neurochem. 159:867. PubMed Lin R, et al. 2022. Nat Methods. 19:976. PubMed Gunner G, et al. 2019. Nat Neurosci. 1.663194444. PubMed Wang L, et al. 2022. Front Cell Neurosci. 15:786020. PubMed Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed de Kleijn KMA, et al. 2022. Biomedicines. 10:. PubMed Wahane S, et al. 2021. Sci Adv. 7:. PubMed
RRID: AB_2650633 (BioLegend Cat. No. 848001) AB_2650634 (BioLegend Cat. No. 848002)
Structure: P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution: Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands. Cellular distribution: cell membrane and cytosol.
Function: G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand/Receptor: ADP and ATP.
Cell Type: Microglia, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: GPCR, Purinergic Receptors
Antigen References: 1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.
Gene ID: 70839
UniProt: View information about P2RY12 on UniProt.org
Clone: S16007D
Regulatory Status: RUO
Other Names: P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype: Rat IgG2b, κ