Biolegend, 848003, PE anti-mouse P2RY12 Antibody, 25μg

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse P2RY12.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research. Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Product Citations: Braun DJ, et al. 2023. PLoS One. 18:e0286495. PubMed Mason HD, et al. 2021. JCI Insight. 6:e149229. PubMed Burns JC, et al. 2021. Bio Protoc. 11:e4091. PubMed Honarpisheh P, et al. 2020. J Neuroinflammation. 0.9625. PubMed Burns JC, et al. 2020. eLife. 9:00. PubMed Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed Milich LM, et al. 2021. J Exp Med. 218:. PubMed Liu Y, et al. 2020. Cell. 183(6):1665-1681.e18. PubMed Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
RRID: AB_2721644 (BioLegend Cat. No. 848003) AB_2721645 (BioLegend Cat. No. 848004)
Structure: P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution: Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands. Cellular distribution: cell membrane and cytosol.
Function: G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand/Receptor: ADP and ATP.
Cell Type: Microglia, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: GPCR, Purinergic Receptors
Antigen References: 1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.
Gene ID: 70839
UniProt: View information about P2RY12 on UniProt.org
Clone: S16007D
Regulatory Status: RUO
Other Names: P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.