Biolegend, 848015, Alexa Fluor® 488 anti-mouse P2RY12 Antibody, 25μg

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse P2RY12.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research. Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.
RRID: AB_2936702 (BioLegend Cat. No. 848015) AB_2936702 (BioLegend Cat. No. 848016)
Structure: P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution: Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands. Cellular distribution: cell membrane and cytosol.
Function: G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand/Receptor: ADP and ATP.
Cell Type: Microglia, Platelets
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: GPCR, Purinergic Receptors
Antigen References: 1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.
Gene ID: 70839
UniProt: View information about P2RY12 on UniProt.org
Clone: S16007D
Regulatory Status: RUO
Other Names: P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype: Rat IgG2b, κ