Biolegend, 848501, Purified anti-Neurogranin, 70-78 Antibody, 25μg

Neurogranin (NRGN) is the main post-synaptic protein kinase substrate that binds to calmodulin (CaM) in the absence of calcium. It is suggested that NRGN is a direct target for thyroid hormone in the human brain, and that the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease.
25μg
Verified Reactivity: Human, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated synthetic peptide corresponding to amino acid residues 70-78 of human neurogranin protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ELISA Capture, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2650691 (BioLegend Cat. No. 848501) AB_2650692 (BioLegend Cat. No. 848502)
Structure: Neurogranin is a 78 amino acid protein with a molecular mass of approximately 7.6 kD.
Distribution: Tissue Distribution: Cerebral cortex neurons in layers II-VI, and pyramidal neurons. Cellular distribution: Golgi apparatus, mitochondria, nucleus, and cytoplasm in neuronal cell body and dendrites.
Function: Neurogranin is a neuron-specific, calmodulin-binding protein that participates in the protein kinase C signaling pathway. Neurogranin is a post-synaptic protein that modulates Ca2+ homeostasis in neurons by binding to and regulating the availability of calmodulin in the absence of Ca2+. Neurogranin function has been implicated in learning and memory.
Ligand/Receptor: Calmodulin.
Cell Type: Neurons
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Postsynaptic proteins
Antigen References: 1. Diez-Guerra FJ. 2010. IUBMB Life. 62(8): 597. (PubMed) 2. De Vos A, et al. 2016. J Alzheimers Dis. 53(4): 1523. (PubMed) 3. De Vos A. et al. 2015. Alzheimers Dement. 11(12):1461. (PubMed)
Gene ID: 4900
UniProt: View information about Neurogranin 70-78 on UniProt.org
Clone: A16007A
Regulatory Status: RUO
Other Names: NRGN, Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng
Isotype: Mouse IgG2b, κ