Biolegend, 848701, Purified anti-Clusterin Antibody, 25μg

Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. The protein exhibits a wide array of biological functions including clearance of cellular debris through chaperone activity, apoptosis, lipid transportation, cell clustering, and complement inhibition. Clusterin is involved in many diseases related to oxidative stress and aging, including neurodegenerative disorders, cancers, diabetes, and inflammatory diseases.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human clusterin.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC-P, Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 20 ng/ml - 3.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2650695 (BioLegend Cat. No. 848701) AB_2650696 (BioLegend Cat. No. 848702)
Structure: Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD.
Distribution: Tissue distribution: Ubiquitously expressed. Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Intracellular clusterin is primarily present in the endoplasmic reticulum, cytosol, mitochondrion, and nucleus.
Function: Clusterin is involved in several biological processes including extra- and intracellular chaperone activity, pro- and anti-apoptotic properties, lipid transportation, cell clustering, modulation of NF-kappa-B transcriptional activity, and complement inhibition.
Interaction: HDL.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation
Molecular Family: Apolipoproteins
Antigen References: 1. Trougakos IP. 2013. Gerontology 59:514. PubMed 2. Rizzi F, Bettuzzi S. 2010. Endocr. Relat. Cancer 17:R1. PubMed 3. Desikan RS, et al. 2014. JAMA Neurol. 71:180. PubMed 4. Carver JA, et al. 2003. IUBMB Life. 55:661. PubMed
Gene ID: 1191
UniProt: View information about Clusterin on UniProt.org
Clone: A15113A
Regulatory Status: RUO
Other Names: Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, Aging-associated gene 4 protein, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2 TRPM-2
Isotype: Mouse IgG2a, κ